Seawater Agar with Horse Blood

[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater Agar with Horse Blood

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Beef extract.................................................................................10.0g
Peptone .......................................................................................10.0g
Seawater.................................................................................750.0mL
Horse blood............................................................................100.0mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse blood, to
tap water and bring volume to 900.0mL. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°–55°C. Aseptically add 100.0mL of sterile horse blood
warmed to 50°–55°C. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation of Aeromonas species.