Seawater Agar Modified (DSMZ Medium 917)

[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater Agar Modified
(DSMZ Medium 917)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................17.7g
Agar ............................................................................................15.0g
MgSO 4 ·7H 2 O..............................................................................4.46g
MgCl 2 ·6H 2 O..................................................................................3.4g
Peptone..........................................................................................2.5g
Hexadecane...................................................................................2.0g
Yeast extract..................................................................................1.5g
KCl..............................................................................................0.48g
Calcium chloride solution........................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Calcium Chloride Solution:
Composition per 10.0mL:
CaCl 2 ·2H 2 O.................................................................................0.98g
Preparation of Calcium Chloride Solution: Add CaCl 2 ·2H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except agar and calci-
um chloride solution, to distilled/deionized water and bring volume to
990.0mL. Adjust pH to 7.2. Mix thoroughly. Add 15.0g agar. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add 10.0mL sterile calcium chloride
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute to
sterile tubes.
Use: For the cultivation and maintenance of Muricauda ruestringensis.