Seawater with Serum

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater with Serum

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................10.0g
Seawater........................................................................................1.0L
Bovine serum, sterile.............................................................100.0mL
Preparation of Medium: Add agar to 1.0L of seawater. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45–50°C. Aseptically add 100.0mL of sterile
liquid beef serum. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the cultivation and maintenance of Basipetospora halophila,
Halosphaeria retorquens, Lagenidium callinectes, and Thraustochy-
trium striatum.