Selenite F Broth

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Selenite F Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

KH 2 PO 4 .........................................................................................7.0g
Pancreatic digest of casein............................................................5.0g
Lactose..........................................................................................4.0g
Na 2 SeO 3 ·5H 2 O..............................................................................4.0g
Na 2 HPO 4 .......................................................................................3.0g
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems .
Caution:  Sodium selenite is toxic and a potential teratogen and care
must be taken to avoid inhalation of the powdered dye, contact with the
skin, or ingestion, especially in pregnant laboratory workers.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat. Do not
autoclave. Distribute into sterile tubes in 10.0mL volumes. Sterilize for
30 min at 0 psi pressure–100°C.
Use: For the isolation and cultivation of Salmonella species from feces,
dairy products, and other specimens.