Selenomonas ruminantium Medium

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Selenomonas ruminantium Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................5.0g
Na 2 CO 3 ........................................................................................4.0g
Sodium acetate..............................................................................4.0g
Yeast extract..................................................................................2.0g
Glucose .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
L -Cysteine·HCl..............................................................................0.5g
Resazurin ...................................................................................1.0mg
n-Valeric acid.............................................................................0.1mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Prepare and dispense medium under
100% CO 2 . Add components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Sparge with 100% CO 2 . Adjust pH to
7.0. Anaerobically distribute into tubes or flasks. Autoclave for 20 min
at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Selenomonas ruminan-
tium and Selenomonas species.