Semisolid IMRV HiVeg Medium Base with Novobiocin

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Semisolid IMRV HiVeg Medium Base
with Novobiocin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate.........................................................................13.5g
Plant peptone...............................................................................13.5g
MnCl 2 ........................................................................................10.91g
Saccharose ....................................................................................7.5g
Agar ..............................................................................................2.7g
KH 2 PO 4 .......................................................................................1.47g
Na 2 S 2 O 3 ........................................................................................0.8g
Lactose..........................................................................................0.5g
FeNH 4 (SO 4 ) 2 ·12H 2 O....................................................................0.2g
Bromcresol Purple......................................................................0.08g
Malachite Green........................................................................0.037g
Novobiocin solution.................................................................. 1.0mL
pH 5.6 ± 0.2 at 25°C
Source: This medium, without novobiocin, is available as a premixed
powder from HiMedia.
Novobiocin Solution:
Composition per 10.0mL:
Novobiocin ...................................................................................0.1g
Novobiocin Solution: Add novobiocin to distilled/deionized water
and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except novobiocin so-
lutiont, to distilled/deionized water and bring volume to 999.0mL. Mix
thoroughly. Gently heat and bring to boiling. Distribute into tubes or
flasks. Do not autoclave. Do not overheat. Cool to 50°C. Aseptically
add 1.0mL novobiocin solution. Pour into sterile Petri dishes or leave
in tubes.
Use:  For the isolation of motile Salmonella spp. from foods