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Semisolid RV HiVeg Medium Base with Novobiocin

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

MnCl2, anhydrous ..................................................................... 10.93g

NaCl ............................................................................................ 7.34g

Plant hydrolysate........................................................................... 4.6g

Plant hydrolysate No. 1................................................................. 4.6g

Agar .............................................................................................. 2.7g

Malachite Green........................................................................ 0.037g

Novobiocin solution.................................................................. 1.0mL

pH 5.4 ± 0.2 at 25°C

Source: This medium, without novobiocin, is available as a premixed

powder from HiMedia.

Novobiocin Solution:

Composition per 10.0mL:

Novobiocin.................................................................................... 0.1g

Preparation of Novobiocin Solution: Add novobiocin to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except novobiocin so

lution, to distilled/deionized water and bring volume to 999.0mL. Mix

thoroughly. Gently heat and bring to boiling. Distribute into tubes or

flasks. Do not autoclave. Do not overheat. Cool to 50°C. Aseptically

add 1.0mL novobiocin solution. Pour into sterile Petri dishes or leave

in tubes.

Use: For the isolation of motile Salmonella spp. from foods