培养基配方

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SF HiVeg Broth

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

SF HiVeg Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate ........................................................................ 20.0g

Glucose ......................................................................................... 5.0g

NaCl.............................................................................................. 5.0g

K2HPO4......................................................................................... 4.0g

KH2PO4......................................................................................... 1.5g

NaN3 ............................................................................................. 0.5g

Bromcresol Purple ...................................................................... 0.032

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of group D enterococci

(Streptococcus faecalis and Streptococcus faecium) from group D non

enterococci and from other Streptococcus species. Group D entero

cocci turn the medium turbid and yellow-brown.