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Shigella HiVeg Broth Base with Novobiocin

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate ........................................................................ 20.0g

NaCl.............................................................................................. 5.0g

K2HPO4......................................................................................... 2.0g

KH2PO4......................................................................................... 2.0g

Glucose ......................................................................................... 1.0g

Polysorbate 80 ........................................................................... 1.5 ml

Novobiocin solution.................................................................11.1mL

pH 7.0 ± 0.2 at 25°C

Source: This medium, without novobiocin, is available as a premixed

powder from HiMedia.

Novobiocin Solution:

Composition per liter:

Novobiocin ................................................................................. 0.05g

Preparation of Novobiocin Solution: Add novobiocin to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except novobiocin so

lution, to distilled/deionized water and bring volume to 988.9mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile no-

vobiocin solution. Mix thoroughly. Aseptically distribute into sterile

tubes.

Use: For the isolation and cultivation of Shigella species from food.