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Simmons’ Citrate Agar (Citrate Agar)

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

Simmons’ Citrate Agar

(Citrate Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

NaCl.............................................................................................. 5.0g

Sodium citrate............................................................................... 2.0g

K2HPO4......................................................................................... 1.0g

(NH4)H2PO4.................................................................................. 1.0g

MgSO4·7H2O................................................................................ 0.2g

Bromthymol Blue ....................................................................... 0.08g

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or

leave in tubes.

Use: For the differentiation of Gram-negative bacteria on the basis of

citrate utilization. Bacteria that can utilize citrate as sole carbon source

turn the medium blue.