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SJ Agar

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

SJ Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

K2HPO4......................................................................................... 1.0g

KCl................................................................................................ 0.5g

MgSO4·7H2O................................................................................ 0.5g

NaNO3 .......................................................................................... 0.5g

FeSO4·7H2O................................................................................ 0.01g

Glucose solution ....................................................................100.0mL

pH 7.2 ± 0.2 at 25°C

Glucose Solution:

Composition per 100.0mL:

D-Glucose...................................................................................... 1.0g

Preparation of Glucose Solution: Add D-glucose to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Medium: Add components, except glucose solu

tion, to distilled/deionized water and bring volume to 900.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile glu

cose solution. Mix thoroughly. Pour into sterile Petri dishes or distrib

ute into sterile tubes.

Use: For the isolation and cultivation of Cytophaga species, Herpeto

siphon species, Saprospira species, and Flexithrix species.