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Skim Milk Horn Meal Mineral Agar

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Bovine horn meal........................................................................ 10.0g

MgSO4·7H2O................................................................................ 0.7g

Ca(NO3)2·4H2O ............................................................................ 0.5g

NaHCO3 ........................................................................................ 0.2g

FeCl3 ..........................................................................................5.0mg

K2HPO4......................................................................................5.0mg

Skim milk solution...................................................................10.0mL

Skim Milk Solution:

Composition per 10.0mL:

Skim milk, powdered.................................................................... 2.5g

Preparation of Skim Milk Solution: Add powdered skim milk to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except skim milk solu

tion, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 60°C. Aseptically add 10.0mL of ster

ile skim milk solution. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the cultivation and maintenance of Capnocytophaga gingi

valis, Capnocytophaga ochracea, and Capnocytophaga sputigena.