培养基配方

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SM Basal Salts Medium

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4....................................................................................... 4.5g

KH2PO4......................................................................................... 1.5g

NH4Cl ........................................................................................... 0.3g

MgSO4·7H2O................................................................................ 0.1g

Trace metals solution .................................................................5.0mL

pH 6.0 ± 0.2 at 25°C

Trace Metals Solution:

Composition per liter:

Ethylenediamine tetraacetate...................................................... 50.0g

ZnSO4·7H2O ............................................................................... 22.0g

CaCl2........................................................................................... 5.54g

MnCl2·4H2O ............................................................................... 5.06g

FeSO4·7H2O................................................................................ 4.99g

CoCl2·H2O .................................................................................. 1.61g

CuSO4·5H2O ............................................................................... 1.57g

(NH4)6Mo7O24·4H2O .................................................................... 1.1g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Adjust pH to 6.0.

Preparation of Medium: Add components to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Thiobacillus delicatus.