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Sodalis glossinidius Medium (DSMZ Medium 1021)

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

Sodalis glossinidius Medium

(DSMZ Medium 1021)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 17.0g

NaCl ............................................................................................. 8.7g

Casein hydrolysate ....................................................................... 8.1g

Yeast extract ................................................................................. 6.2g

D-Glucose ..................................................................................... 5.0g

KCl ............................................................................................. 0.25g

CaCl2·2H2O................................................................................. 0.25g

NaHCO3 ...................................................................................... 0.15g

MgCl2·6H2O................................................................................ 0.12g

Phosphate solution .................................................................100.0mL

pH 8.0 ± 0.2 at 25°C

Phosphate Solution:

Composition per 100.0mL:

NaH2PO4 ..................................................................................... 0.28g

Preparation of Phosphate Solution: Add NaH2PO4 to distilled/

deionized water and bring volume to 100.0mL. Mix thoroughly. Auto

clave or 20 min at 15 psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Add components, except phosphate solu

tion, to distilled/deionized water and bring volume to 900.0L. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 20 min at

15 psi pressure–121°C. Cool to 50°C. Aseptically add phosphate solu

tion. Mix thoroughly. Adjust pH to 8.0. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the cultivation of Sodalis glossinidius.