培养基配方

您现在的位置：网站首页 >> 培养基配方

Soft Agar Gelatin Overlay

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per plate:

Base agar..................................................................................15.0mL

Soft agar gelatin overlay ............................................................2.5mL

pH 7.0 ± 0.2 at 25°C

Base Agar:

Composition per liter:

Agar ............................................................................................ 15.0g

Peptone.......................................................................................... 5.0g

NaCl .............................................................................................. 5.0g

Beef extract ................................................................................... 3.0g

MnSO4·H2O ................................................................................ 0.05g

Preparation of Base Agar: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C.

Soft Agar Gelatin Overlay:

Composition per liter:

Gelatin......................................................................................... 15.0g

Agar .............................................................................................. 8.0g

Peptone.......................................................................................... 5.0g

NaCl .............................................................................................. 5.0g

Beef extract ................................................................................... 3.0g

MnSO4·H2O ................................................................................ 0.05g

Preparation of Soft Agar Gelatin Overlay: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C.

Preparation of Medium: Aseptically pour cooled, sterile base agar

into sterile Petri dishes in 15.0mL volumes. Allow agar to solidify. In

oculate plates with samples. Overlay each plate with 2.5mL of soft agar

gelatin overlay.

Use: For the cultivation and differentiation of microorganisms based

on proteolytic activity.