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Soil Extract Glycerol Medium

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glycerol ...................................................................................... 20.0g

Peptone.......................................................................................... 5.0g

Beef extract ................................................................................... 3.0g

Soil extract .............................................................................150.0mL

pH 7.0 ± 0.2 at 25°C

Soil Extract:

Composition per liter:

Soil ............................................................................................ 400.0g

Preparation of Soil Extract: Allow garden soil to air dry. Add

400.0g of the air-dried garden soil to 960.0mL of tap water. Autoclave

for 60 min at 15 psi pressure–121°C. Allow to cool to 25°C. Let stand

until settling ceases. Decant the liquid through Whatman filter paper.

Autoclave for 30 min at 15 psi pressure–121°C. Cool to room temepra

ture. Let stand until settling ceases.

Preparation of Medium: Add components to tap water and bring

volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Mycobacterium terrae.