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SS Deoxycholate Agar (Salmonella Shigella Deoxycholate Agar) (SSDC)

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

SS Deoxycholate Agar

(Salmonella Shigella Deoxycholate Agar)

(SSDC)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 13.5g

Lactose........................................................................................ 10.0g

Sodium deoxycholate.................................................................. 10.0g

Bile salts........................................................................................ 8.5g

Na2S2O3 ........................................................................................ 8.5g

Sodium citrate............................................................................... 8.5g

Beef extract................................................................................... 5.0g

Pancreatic digest of casein............................................................ 2.5g

Peptic digest of animal tissue ....................................................... 2.5g

CaCl2·2H2O .................................................................................. 1.0g

Ferric citrate.................................................................................. 1.0g

Neutral Red............................................................................... 0.025g

Brilliant Green ......................................................................... 0.33mg

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Do not autoclave. Cool to 45°–50°C. Pour

into sterile Petri dishes in 20.0mL volumes. Allow the surface of the

plates to dry before inoculation.

Use: For the isolation and cultivation of Yersinia enterocolitica from

foods.