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Standard II Nutrient Agar

[所属分类:培养基配方] [发布时间:2021-8-25] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 13.0g

Tryptose ........................................................................................ 7.0g

NaCl .............................................................................................. 5.0g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of nonfastidious microorganisms. For the

maintenance of cultures of a wide variety of nonfastidious bacteria.

May also be used as a base for blood and other enrichments for the cul

tivation of fastidious microorganisms. May be used to determine

indole production.