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Staphylococcus Broth (m-Staphylococcus Broth)

[所属分类:培养基配方] [发布时间:2021-8-25] [发布人:网站管理员2] [阅读次数:] [返回]

Staphylococcus Broth

(m-Staphylococcus Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................ 75.0g

Mannitol...................................................................................... 10.0g

Pancreatic digest of casein.......................................................... 10.0g

K2HPO4......................................................................................... 5.0g

Yeast extract.................................................................................. 2.5g

Lactose.......................................................................................... 2.0g

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and enumeration of pathogenic and enterotox

igenic staphylococci by the membrane filter method. Also, when used

in conjunction with Lipovitellin-salt-mannitol agar, for the detection of

Staphylococcus aureus in swimming pool water.