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Staphylococcus/Streptococcus Selective Medium

[所属分类:培养基配方] [发布时间:2021-8-25] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1060.0mL:

Columbia blood agar base.............................................................1.0L

Horse blood, defibrinated ........................................................50.0mL

Antibiotic inhibitor ..................................................................10.0mL

pH 7.3 ± 0.2 at 25°C

Columbia Blood Agar Base:

Composition per liter:

Special peptone ........................................................................... 23.0g

Agar ............................................................................................ 10.0g

NaCl .............................................................................................. 5.0g

Starch ............................................................................................ 1.0g

Source: Columbia blood agar base is available as a premixed powder

from Oxoid Unipath.

Preparation of Columbia Blood Agar Base: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C.

Antibiotic Inhibitor:

Composition per 10.0mL:

Nalidixic acid............................................................................ 0.015g

Colistin sulfate ............................................................................ 0.01g

Ethanol (95% solution) ............................................................10.0mL

Preparation of Antibiotic Inhibitor: Add components to 10.0mL

ethanol. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 1.0L of cooled sterile Columbia blood

agar base, aseptically add sterile horse blood and sterile antibiotic in

hibitor. Mix thoroughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the selective isolation of Staphylococcus aureus and strepto

cocci from clinical specimens or foods.