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Starch Agar

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

Starch Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Potato starch................................................................................ 10.0g

Pancreatic digest of gelatin........................................................... 5.0g

Beef extract................................................................................... 3.0g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components, except potato starch, to

distilled/deionized water and bring volume to 500.0mL. Mix thorough

ly. Gently heat and bring to boiling. Add potato starch to distilled/de

ionized water and bring volume to 250.0mL. Gently heat and bring to

boiling. Combine the two solutions and bring the volume to 1.0L with

distilled/deionized water. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes.

Use: For the cultivation and differentiation of aerobic Actinomyces

species based on amylase production. After incubation, starch hydro

lysis is determined by the addition of Gram’s or Lugol’s iodine solu

tion. Organisms that produce amylase appear as colonies surrounded

by a clear zone.