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Starch Salts Agar

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

Starch Salts Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Solution A..............................................................................500.0mL

Solution B ..............................................................................500.0mL

Solution A:

Composition per 500.0mL:

CaCO3 ........................................................................................... 2.0g

(NH4)2SO4 .................................................................................... 2.0g

K2HPO4......................................................................................... 1.0g

MgSO4·7H2O................................................................................ 1.0g

NaCl.............................................................................................. 1.0g

Trace salts ..................................................................................1.0mL

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Trace Salts:

Composition per 100.0mL:

FeSO4·7H2O.................................................................................. 0.1g

MnCl2·4H2O ................................................................................. 0.1g

ZnSO4·7H2O ................................................................................. 0.1g

Preparation of Trace Salts: Add components to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave

for 15 min at 15 psi pressure–121°C.

Solution B:

Composition per 500.0mL:

Soluble starch.............................................................................. 10.0g

Preparation of Solution B: Make a paste of the starch with a small

volume of distilled/deionized water and then gradually add the starch

to distilled/deionized water and bring volume to 500.0mL. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Thoroughly mix 500.0mL of solution A

and 500.0mL of solution B. Adjust the pH to 7.2. Add 20.0g of agar

and gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Thoroughly mix to evenly distribute

the precipitate. Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the cultivation of Amycolatopsis rugosa, Nocardia lucida, Strep

tomyces canus, Streptomyces cyanogriseus, Streptomyces fradiae, Strep

tomyces hiroshimensis, Streptomyces kuwaitiensis, Streptomyces rubro

verrucosus, Streptomyces spinoverrucosus, Streptomyces viridover

rucosus, and Thermoactinomyces dichotomicus.

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Starch Salts Agar