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Starkey’s Medium C, Modified with Salt

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 25.0g

Sodium lactate............................................................................... 3.5g

MgSO4·7H2O ................................................................................ 2.0g

Na2SO4 .......................................................................................... 1.0g

NH4Cl ........................................................................................... 1.0g

Yeast extract.................................................................................. 1.0g

KH2PO4......................................................................................... 0.5g

CaCl2·2H2O................................................................................... 0.1g

Ferrous ammonium sulfate solution ........................................50.0mL

L-Cysteine·HCl·H2O solution ..................................................10.0mL

pH 7.5 ± 0.2 at 25°C

Ferrous Ammonium Sulfate Solution:

Composition per 100.0mL:

Fe(NH4)2(SO4)2·6H2O .................................................................. 1.0g

Preparation of Ferrous Ammonium Sulfate Solution: Add

Fe(NH4)2(SO4)2·6H2O to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly. Filter sterilize.

L-Cysteine·HCl·H2O Solution:

Composition per 10.0mL:

L-Cysteine·HCl·H2O ................................................................... 0.75g

Preparation of L-Cysteine·HCl·H2O Solution: Add L-cyste

ine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL.

Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except ferrous ammo

nium sulfate solution and L-cysteine·HCl·H2O solution, to tap water

and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add 50.0mL of sterile ferrous ammonium sul

fate solution and 10.0mL of sterile L-cysteine·HCl·H2O solution. Mix

thoroughly. Adjust pH to 7.5 with filter-sterilized 2N NaOH. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of halophilic Desulfovibrio

species.