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Stock Culture Agar

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

Stock Culture Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart infusion.................................................................... 500.0g

Gelatin......................................................................................... 10.0g

Proteose peptone ......................................................................... 10.0g

Agar .............................................................................................. 7.5g

Casein............................................................................................ 5.0g

Na2HPO4 ....................................................................................... 4.0g

Sodium citrate ............................................................................... 3.0g

Glucose ......................................................................................... 0.5g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to cold distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly. Gently heat

while stirring and bring to boiling. Distribute into tubes or flasks. Au

toclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri

dishes or leave in tubes.

Use: For the maintenance of pathogenic and nonpathogenic bacteria,

especially streptococci.