编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

Sulfurospirillum MV Medium (DSMZ Medium 1097)

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

Sulfurospirillum MV Medium

(DSMZ Medium 1097)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ........................................................................................... 20.0g

MgCl2·6H2O.................................................................................. 3.0g

CaCl2·2H2O................................................................................... 0.8g

KCl ............................................................................................... 0.7g

NH4Cl .......................................................................................... 0.2g

KH2PO4......................................................................................... 0.2g

Resazurin ..................................................................................0.5mg

Vitamin solution.......................................................................10.0mL

Sulfide solution ........................................................................10.0mL

Fumarate solution ....................................................................10.0mL

Bicarbonate solution ................................................................10.0mL

Trace elements solution SL-10 ..................................................2.0mL

Selenite-tungstate solution.........................................................1.0mL

pH 7.2 ± 0.2 at 25°C

Bicarbonate Solution:

Composition per 10.0mL:

NaHCO3 ....................................................................................... 2.5g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge

with 20% CO2 + 80% N2. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to room temperature.

Vitamin Solution:

Composition per liter:

Pyridoxine-HCl........................................................................ 10.0mg

Thiamine-HCl·2H2O.................................................................. 5.0mg

Riboflavin .................................................................................. 5.0mg

Nicotinic acid............................................................................. 5.0mg

D-Ca-pantothenate ..................................................................... 5.0mg

p-Aminobenzoic acid................................................................. 5.0mg

Lipoic acid ................................................................................. 5.0mg

Biotin ......................................................................................... 2.0mg

Folic acid ................................................................................... 2.0mg

Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Sparge

with 100% N2. Filter sterilize.

Fumarate Solution:

Composition per 10.0mL:

Na2-fumarate ................................................................................ 1.6g

Preparation of Fumarate Solution: Add Na2-fumarate to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to room temperature.

Trace Elements Solution SL-10:

Composition per liter:

MgSO4·7H2O................................................................................ 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl.............................................................................................. 1.0g

MnSO4·2H2O................................................................................ 0.5g

CoSO4·7H2O............................................................................... 0.18g

ZnSO4·7H2O ............................................................................... 0.18g

CaCl2·2H2O .................................................................................. 0.1g

FeSO4·7H2O.................................................................................. 0.1g

NiCl2·6H2O............................................................................... 0.025g

KAl(SO4)2·12H2O....................................................................... 0.02g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·4H2O .......................................................................... 0.01g

CuSO4·5H2O............................................................................... 0.01g

Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution SL-10: Add nitrilotri

acetic acid to 500.0mL of distilled/deionized water. Dissolve by adjust

ing pH to 6.5 with KOH. Add remaining components. Add distilled/

deionized water to 1.0L. Mix thoroughly. Adjust pH to 7.0.

Selenite-Tungstate Solution:

Composition per liter:

NaOH............................................................................................ 0.5g

Na2WO4·2H2O........................................................................... 4.0mg

Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Selenite-Tungstate Solution: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Sulfide Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 0.3g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave

under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room

temperature.

Preparation of Medium: Add components, except vitamin solution,

bicarbonate solution, fumarate solution, and sulfide solution, to distilled/

deionized water and bring volume to 960.0mL. Mix thoroughly. Gently

heat and bring to boiling. Boil for 1 min. Cool to room temperature un

der 80% N2 + 20% CO2. Dispense under same gas atmosphere into cul

ture vessels. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically

add fumarate, sulfide, vitamin, and bicarbonate solutions. Adjust the

pH to 7.2.

Use: For the cultivation of Sulfurospirillum spp.

在线客服

Sulfurospirillum MV Medium (DSMZ Medium 1097)