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Super MMB Medium (LMG Medium 188)

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

Super MMB Medium

(LMG Medium 188)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Yeast extract.................................................................................. 1.0g

KH2PO4......................................................................................... 1.0g

Peptone.......................................................................................... 0.4g

Sodium succinate .......................................................................... 0.4g

NH4Cl ........................................................................................... 0.2g

NaCl .............................................................................................. 0.2g

MgSO4·7H2O ................................................................................ 0.2g

CaCl2·2H2O..............................................................................10.0mg

Ferric citrate...............................................................................5.0mg

Vitamin solution.......................................................................20.0mL

Trace elements solution SL-6 ...................................................1.0mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution SL-6 :

Composition per liter:

H3BO3 ........................................................................................... 0.3g

CoCl2·6H2O .................................................................................. 0.2g

ZnSO4·7H2O ................................................................................. 0.1g

MnCl2·4H2O................................................................................ 0.03g

Na2MoO4·H2O ............................................................................ 0.03g

NiCl2·6H2O ................................................................................. 0.02g

CuCl2·2H2O................................................................................. 0.01g

Preparation ofTrace Elements Solution SL-6 : Add compo

nents to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Adjust pH to 3.4.

Vitamin Solution:

Composition per liter:

Calcium DL-pantothenate...........................................................5.0mg

Riboflavin ..................................................................................5.0mg

Thiamine·HCl ............................................................................5.0mg

Biotin .........................................................................................2.0mg

Folic acid....................................................................................2.0mg

Vitamin B12 ................................................................................0.1mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except vitamin solu

tion, to 980.0mL distilled/deionized water. Mix thoroughly. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add

20.0mL sterile vitamin solution. Mix thoroughly. Aseptically distribute

to sterile tubes or flasks.

Use: For the cultivation of Aquabacter spp.

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Super MMB Medium (LMG Medium 188)