培养基配方

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Synthetic Seawater Medium

[所属分类:培养基配方] [发布时间:2021-9-1] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................ 27.0g

MgSO4·7H2O................................................................................ 7.0g

Monosodium glutamate ................................................................ 5.0g

Tris(hydroxymethyl)aminomethane buffer................................... 2.0g

Glucose ......................................................................................... 1.0g

KCl................................................................................................ 0.6g

CaCl2............................................................................................. 0.3g

Sodium glycerophosphate............................................................. 0.2g

Vitamin B12 .................................................................................1.0μg

pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation and maintenance of Leucothrix mucor.