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Tarshis Blood Agar

[所属分类:培养基配方] [发布时间:2021-9-1] [发布人:网站管理员2] [阅读次数:] [返回]

Tarshis Blood Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

Beef heart infusion.................................................................... 500.0g

Agar ............................................................................................ 15.0g

Meat peptone .............................................................................. 10.0g

NaCl.............................................................................................. 5.0g

Penicillin G, sterile............................................................... 100,000U

Sheep blood, sterile................................................................300.0mL

Glycerol ...................................................................................10.0mL

pH 6.6 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood and

penicillin G, to distilled/deionized water and bring volume to 750.0mL.

Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile

sheep blood and sterile penicillin G. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Mycobacterium tuberculosis.