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TAT Broth Base (Trypticase™ Azolectin Tween™ Broth Base)

[所属分类:培养基配方] [发布时间:2021-9-1] [发布人:网站管理员2] [阅读次数:] [返回]

TAT Broth Base

(Trypticase™ Azolectin Tween™ Broth Base)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 20.0g

Lecithin......................................................................................... 5.0g

Polysorbate 20 (Tween™ 20) ..................................................40.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add pancreatic digest of casein and leci

thin to distilled/deionized water and bring volume to 960.0mL. Add the

Tween™ 20. Mix thoroughly. Gently heat and bring to 48°–50°C for

30 min. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the isolation of Gram-negative organisms from topical drugs

and cosmetics.