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TCBS HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

TCBS HiVeg Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................ 20.0g

Agar ............................................................................................ 15.0g

Plant peptone No. 3..................................................................... 15.0g

NaCl............................................................................................ 10.0g

Sodium citrate............................................................................. 10.0g

Na2S2O3 ...................................................................................... 10.0g

Yeast extract.................................................................................. 6.0g

Synthetic detergent No. II............................................................. 2.0g

Ferric citrate.................................................................................. 1.0g

Thymol Blue ............................................................................... 0.04g

Bromthymol Blue ....................................................................... 0.04g

pH 8.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Do not autoclave. Cool to 45°–50°C. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of Vibrio cholerae and Vibrio para

haemolyticus from a variety of clinical and nonclinical specimens. For

the cultivation of enteropathogenic vibrios causing food poisoning.