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TDN Broth (DSMZ Medium 1012)

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

TDN Broth

(DSMZ Medium 1012)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin........................................................... 0.5g

Casamino acids ............................................................................ 0.5g

Beef extract................................................................................... 0.3g

Yeast extract ................................................................................. 0.1g

Magnesium chloride solution ..................................................10.0mL

Calcium chloride solution........................................................10.0mL

pH 7.2 ± 0.2 at 25°C

Calcium Chloride Solution:

Composition per 10.0mL:

CaCl2·2H2O .................................................................................. 0.3g

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thor-

oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room

temperature.

Magnesium Chloride Solution:

Composition per 10.0mL:

MgCl2·6H2O.................................................................................. 0.6g

Preparation of Magnesium Chloride Solution: Add MgCl2·6H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room

temperature.

Preparation of Medium: Add components, except calcium chlo

ride and magnesium chloride solutions, to distilled/deionized water

and bring volume to 980.0mL. Mix thoroughly. Adjust the pH to 7.2

with NaOH. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to room temperature. Aspetically add cal

cium chloride and magnesium chloride solutions. Mix thoroughly.

Use: For the cultivation of Bdellovibrio bacteriovorus.