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Tech Agar

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

Tech Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of gelatin......................................................... 20.0g

Agar ............................................................................................ 13.6g

K2SO4·7H2O ............................................................................... 10.0g

MgCl2·6H2O ................................................................................. 1.4g

Glycerol ...................................................................................10.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components, except glycerol, to dis

tilled/deionized water and bring volume to 990.0mL. Mix thoroughly.

Add glycerol. Gently heat and bring to boiling. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile

Petri dishes or leave in tubes.

Use: For the production of pyocyanin pigment by Pseudomonas spe

cies.