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Tellurite Glycine Agar

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 17.5g

Pancreatic digest of casein.......................................................... 10.0g

Glycine........................................................................................ 10.0g

Yeast extract.................................................................................. 6.5g

D-Mannitol..................................................................................... 5.0g

K2HPO4......................................................................................... 5.0g

LiCl ............................................................................................... 5.0g

Enzymatic hydrolysate of soybean meal ...................................... 3.5g

Chapman tellurite solution.......................................................10.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Caution: Lithium chloride is harmful. Avoid bodily contact and inha

lation of vapors. On contact with skin wash with plenty of water imme

diately.

Chapman Tellurite Solution:

Composition per 100.0mL:

K2TeO3 .......................................................................................... 1.0g

Preparation of Chapman Tellurite Solution: Add K2TeO3 to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly. Filter sterilize.

Caution: Potassium tellurite is toxic.

Preparation of Medium: Add components, except Chapman tellu

rite solution, to distilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL of

sterile Chapman tellurite solution. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes. Allow the surface of the plates to

dry before inoculating.

Use: For the isolation and cultivation of coagulase-positive staphylo

cocci.