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TEP Uric Acid Medium

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Na2HPO4·12H2O........................................................................... 9.0g

Uric acid........................................................................................ 4.0g

Pancreatic digest of casein............................................................ 1.7g

KH2PO4......................................................................................... 1.5g

NaCl.............................................................................................. 0.5g

Papaic digest of soybean meal...................................................... 0.3g

K2HPO4....................................................................................... 0.25g

Glucose ....................................................................................... 0.25g

MgSO4·7H2O................................................................................ 0.2g

CaCl2........................................................................................... 0.02g

Ferric ammonium citrate............................................................ 1.2mg

MnCl2·4H2O .............................................................................. 1.0mg

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Bacillus fastidiosus and

other microorganisms that can utilize uric acid as a carbon source.