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培养基配方

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Teredinobacter Medium

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition 1010.0mL:

Solution A................................................................................50.0mL

Solution C ..............................................................................750.0mL

Solution B ..............................................................................200.0mL

Solution D................................................................................10.0mL

Solution A:

Composition per 50.0mL:

K2HPO4....................................................................................20.0mg

Na2CO3 ...................................................................................20.0mg

Ferric ammonium citrate............................................................6.0mg

EDTA .........................................................................................1.0mg

Trace metal mix A5....................................................................1.0mL

Trace Metal Mix A5:

Composition per liter:

H3BO3 ......................................................................................... 2.86g

MnCl2·4H2O................................................................................ 1.81g

ZnSO4·7H2O ............................................................................. 0.222g

Na2MoO4·2H2O .......................................................................... 0.39g

CuSO4·5H2O ............................................................................. 0.079g

Co(NO3)2·6H2O .......................................................................49.4mg

Preparation of Trace Metal Mix A5: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°–55°C.

Solution B:

Composition per 200.0mL:

Agar, noble.................................................................................... 2.0g

Preparation of Solution B: Add agar to distilled/deionized water

and bring volume to 200.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

50°–55°C.

Solution C:

Composition per 750.0mL:

Seawater.................................................................................750.0mL

Preparation of Solution C: Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 50°–55°C.

Solution D:

Composition per 10.0mL:

Cellulose, Sigmacell Type 101 ..................................................... 1.0g

Preparation of Solution D: Add cellulose to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically combine 50.0mL of sterile

solution A, 200.0mL of sterile solution B, 750.0mL of sterile solution

C, and 10.0mL of sterile solution D. Mix thoroughly. Aseptically dis

tribute into sterile tubes.

Use: For the cultivation of unidentified bacterium ATCC 39867.

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Teredinobacter Medium