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Termitobacter Medium

[所属分类:培养基配方] [发布时间:2021-9-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1060.0mL

NH4Cl ........................................................................................... 1.0g

NaCl.............................................................................................. 0.6g

Sodium acetate·3H2O.................................................................... 0.5g

L-Cysteine·HCl ............................................................................. 0.5g

K2HPO4......................................................................................... 0.3g

KH2PO4......................................................................................... 0.3g

MgCl2·6H2O ................................................................................. 0.2g

CaCl2·2H2O .................................................................................. 0.1g

KCl................................................................................................ 0.1g

Yeast extract.................................................................................. 0.2g

Resazurin ................................................................................... 0.5mg

NaHCO3 solution .....................................................................40.0mL

Na2S·9H2O solution.................................................................10.0mL

Trans-3,4,5-trimethoxycinnamate solution..............................10.0mL

Trace elements solution SL-10 ..................................................1.5mL

NaHCO3 Solution:

Composition per 10.0mL:

NaHCO3........................................................................................ 1.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Sparge

with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–

121°C.

Na2S·9H2O Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Before use, neutralize to pH 7.0 with sterile HCl.

Trans-3,4,5-Trimethoxycinnamate Solution:

Composition per 10.0mL:

Trans-3,4,5-trimethoxycinnamate............................................... 1.19g

Preparation of Trans-3,4,5-Trimethoxycinnamate Solution:

Add trans-3,4,5-trimethoxycinnamate to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Neu

tralize with NaOH. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Prepare and dispense medium under 80%

N2 + 20% CO2 gas mixture. Add components, except NaHCO3 solu

tion, trans-3,4,5-trimethoxycinnamate solution, and Na2S·9H2O solu

tion, to distilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Sparge with 80% N2 + 20% CO2 gas mixture. Autoclave

for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add

40.0mL of sterile NaHCO3 solution, 10.0mL of sterile trans-3,4,5-

trimethoxycinnamate solution, and 10.0mL of sterile Na2S·9H2O solu

tion. Mix thoroughly. Aseptically and anaerobically distribute into

sterile tubes or bottles.

Use: For the cultivation of Sporobacter termitidis.

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Termitobacter Medium