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Terrific Broth with 200 μg/ml Ampicillin (ATCC Medium 1945)

[所属分类:培养基配方] [发布时间:2021-9-3] [发布人:网站管理员2] [阅读次数:] [返回]

Terrific Broth with 200 μg/ml Ampicillin

(ATCC Medium 1945)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................ 24.0g

Tryptone...................................................................................... 12.0g

Glycerol ...................................................................................... 12.0g

Buffer solution .......................................................................100.0mL

Ampicillin solution ..................................................................10.0mL

pH 7.2 ± 0.2 at 25°C

Buffer Solution:

Composition per 100.0mL:

KH2PO4....................................................................................... 2.31g

K2HPO4..................................................................................... 12.54g

Preparation of Buffer Solution: Add components to 100.0mL

distilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 25°C.

Ampicillin Solution:

Composition per 10.0mL:

Ampicillin ...............................................................................200.0μg

Preparation of Ampicillin Solution: Add ampicillin to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except buffer and am

picillin solutions, to 890.0mL distilled/deionized water. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Aseptically add the buffer and ampicillin solutions. Mix thoroughly.

Aseptically distribute to tubes or flasks.

Use: For the cultivation of plasmid-bearing strains of Escherichia coli.

For the cloning of pSVbeta, pCMVbeta, pTKbeta, pADbeta, pNASS

beta, and pBS-hTOP2 plasmids in E. coli.