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Tetracycline Luria Agar No. 3

[所属分类:培养基配方] [发布时间:2021-9-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of casein.......................................................... 10.0g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 0.5g

Glucose solution ......................................................................20.0mL

Tetracycline solution................................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Glucose Solution:

Composition per 50.0mL:

Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril

ize.

Tetracycline Solution:

Composition per 10.0mL:

Tetracycline..............................................................................20.0mg

Preparation of Tetracycline Solution: Add tetracycline to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except tetracycline solu

tion and glucose solution, to distilled/deionized water and bring volume to

970.0mL. Mix thoroughly. Bring pH to 7.0. Gently heat and bring to boil

ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Aseptically add 10.0mL of sterile tetracycline solution and 20.0mL of ster

ile glucose solution. Mix thoroughly. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the cultivation of Escherichia coli.