培养基配方

您现在的位置：网站首页 >> 培养基配方

Tetrathionate Broth with Novobiocin

[所属分类:培养基配方] [发布时间:2021-9-3] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2S2O3 ...................................................................................... 38.0g

CaCO3 ......................................................................................... 25.0g

Casein/meat peptone (50/50) ...................................................... 18.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 2.0g

D-Mannitol.................................................................................... 0.5g

Glucose ......................................................................................... 0.5g

Sodium deoxycholate.................................................................... 0.5g

Brilliant Green ............................................................................ 0.01g

Novobiocin ................................................................................ 4.0mg

Iodine solution .........................................................................40.0mL

pH 7.5–7.8 at 25°C

Iodine Solution:

Composition per 40.0mL:

KI .................................................................................................. 8.0g

Iodine ............................................................................................ 5.0g

Preparation of Iodine Solution: Add iodine and KI to distilled/

deionized water and bring volume to 40.0mL. Mix thoroughly.

Preparation of Medium: Add components, except iodine solution,

to distilled/deionized water and bring volume to 960.0mL. Mix thor

oughly. Gently heat and bring to boiling. Do not autoclave. Cool to

40°C. Add 40.0mL of iodine solution. Mix thoroughly. Distribute into

tubes in 10.0mL volumes. Use medium the same day it is prepared.

Use: For the isolation of Salmonella species, except Salmonella typhi,

and Arizona species from fecal specimens and other specimens of san

itary importance. Novobiocin suppresses the growth of Proteus spe

cies.