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TGMM-2 Medium

[所属分类:培养基配方] [发布时间:2021-9-6] [发布人:网站管理员2] [阅读次数:] [返回]

TGMM-2 Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Saccharomyces cerevisiae

type II cells, dried................................................................... 3.0g

Soy lecithin penterythritol mix ..................................................... 0.1g

Skim milk powder...................................................................... 1.0mg

Source: Dried Saccharomyces cerevisiae type II cells and soy lecithin

(type II-S L-α-phosphatidyl choline) are available from Sigma Chemi

cal Co.

Soy Lecithin Penterythritol Mix:

Composition per 0.11g:

Penterythritol ................................................................................ 0.1g

Soy lecithin (type II-S

L-α-phosphatidyl choline)................................................. 10.0mg

Preparation of Soy Lecithin Penterythritol Mix: Mix soy lec

ithin with the inert solid diluent penterythritol as a 1:10 mixture and

grind to a fine powder.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat to 85°C

while vigorously mixing for 10 min. Distribute 10.0mL volumes into

tubes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Tetrahymena limacis, Tetrahymena patula,

Tetrahymena rostrata, and Tetrahymena setosa.