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Thermoactinomyces Medium (DSMZ Medium 978)

[所属分类:培养基配方] [发布时间:2021-9-6] [发布人:网站管理员2] [阅读次数:] [返回]

Thermoactinomyces Medium

(DSMZ Medium 978)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Polypeptone™ ............................................................................ 30.0g

Agar ............................................................................................ 15.0g

Glycerol ........................................................................................ 2.0g

L-Asparagine................................................................................. 1.0g

K2HPO4......................................................................................... 1.0g

Vitamin B solution...................................................................10.0mL

Trace salts solution ....................................................................1.0mL

pH 7.2 ± 0.2 at 25°C

Trace Salts Solution:

Composition per 100.0mL:

FeSO4·7H2O.................................................................................. 0.1g

MnCl2·4H2O ................................................................................. 0.1g

ZnSO4·7H2O ................................................................................. 0.1g

Preparation of Trace Salts Solution: Add components to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Vitamin B Solution:

Composition per 200.0mL:

Thiamine-HCl.......................................................................... 10.0mg

Riboflavin ................................................................................ 10.0mg

Nicotinate................................................................................. 10.0mg

Pyridoxine-HCl........................................................................ 10.0mg

Inositol .....................................................................................10.0mg

Calcium pantothenate ..............................................................10.0mg

p-Aminobenzoate.....................................................................10.0mg

D-Biotin......................................................................................5.0mg

Preparation of Vitamin B Solution: Add components to distilled/

deionized water and bring volume to 200.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except vitamin B solu

tion, to distilled/deionized water and bring volume to 900.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

room temperature. Aseptically add 100.0mL vitamin B solution. Asep

tically distribute into sterile tubes or flasks.

Use: For the cultivation of Thermoactinomyces peptonophilus.