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Thermoactinomyces Medium (DSMZ Medium 978)

[所属分类:培养基配方] [发布时间:2021-9-6] [发布人:网站管理员2] [阅读次数:] [返回]

Thermoactinomyces Medium

(DSMZ Medium 978)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Polypeptone ................................................................................ 30.0g

Agar ........................................................................................... 15.0g

Glycerol ........................................................................................ 2.0g

L-Asparagine................................................................................. 1.0g

KH2PO4......................................................................................... 1.0g

Vitamin solution.......................................................................10.0mL

Trace elements solution .............................................................1.0mL

pH 7.2 ± 0.2 at 25°C

Vitamin Solution:

Composition per 200.0mL:

Pyridoxine-HCl........................................................................10.0mg

Thiamine-HCl·2H2O................................................................10.0mg

Riboflavin ................................................................................10.0mg

Nicotinic acid...........................................................................10.0mg

D-Ca-pantothenate....................................................................10.0mg

Inositol .....................................................................................10.0mg

p-Aminobenzoic acid...............................................................10.0mg

Biotin .........................................................................................5.0mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 200.0mL. Mix thoroughly. Filter

sterilize.

Trace Elements Solution:

Composition per 100.0mL:

FeSO4·7H2O.................................................................................. 0.1g

MnCl2·4H2O.................................................................................. 0.1g

ZnSO4·7H2O ................................................................................. 0.1g

Preparation of Trace Elements Solution: Add components to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components, except vitamin solu

tion, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Adjust the pH to 7.2. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to room temperature. Aspetically add vitamin solu

tion. Mix thoroughly. Aseptically dispense into culture vessels.

Use: For the cultivation of Thermoactinomyces spp.