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Thermoanaerobacter tengcongensis Medium (DSMZ Medium 965)

[所属分类:培养基配方] [发布时间:2021-9-6] [发布人:网站管理员2] [阅读次数:] [返回]

Thermoanaerobacter tengcongensis

Medium

(DSMZ Medium 965)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Soluble starch.............................................................................. 10.0g

NaCl.............................................................................................. 2.0g

Tryptone........................................................................................ 2.0g

NH4Cl ........................................................................................... 1.0g

Yeast extract.................................................................................. 1.0g

MgCl2·6H2O ................................................................................. 0.5g

Cysteine-HCl·H2O ........................................................................ 0.5g

K2HPO4......................................................................................... 0.3g

KH2PO4......................................................................................... 0.3g

KCl................................................................................................ 0.2g

CaCl2·2H2O ................................................................................ 0.05g

Resazurin ................................................................................... 0.5mg

Thiosulfate solution .................................................................50.0mL

Trace elements solution ...........................................................10.0mL

pH 7.5 ± 0.2 at 25°C

Thiosulfate Solution:

Composition per 50.0mL:

Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Thiosulfate Solution: Add Na2S2O3·5H2O to dis

tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper

ature.

Trace Elements Solution:

Composition per liter:

MgSO4·7H2O................................................................................ 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl.............................................................................................. 1.0g

MnSO4·2H2O................................................................................ 0.5g

CoSO4·7H2O............................................................................... 0.18g

ZnSO4·7H2O ............................................................................... 0.18g

CaCl2·2H2O .................................................................................. 0.1g

FeSO4·7H2O.................................................................................. 0.1g

NiCl2·6H2O............................................................................... 0.025g

KAl(SO4)2·12H2O....................................................................... 0.02g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·4H2O .......................................................................... 0.01g

CuSO4·5H2O............................................................................... 0.01g

Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH

to 6.5 with KOH. Add remaining components. Add distilled/deionized

water to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except thiosulfate so

lution, to distilled/deionized water and bring volume to 950.0mL. Mix

thoroughly. Gently heat and bring to boiling. Boil for 3 min. Cool to

room temperature while sparging with 100% N2 gas. Adjust pH to 7.5.

Distribute into tubes or bottles under 100% N2 gas. Autoclave for 15

min at 15 psi pressure–121°C. Cool to room temperature. Aseptically

and anaerobically under 100% N2 gas, add 50.0mL sterile thiosulfate

solution per liter of medium.

Use: For the cultivation of Thermoanaerobacter tengcongensis.

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Thermoanaerobacter tengcongensis Medium (DSMZ Medium 965)