培养基配方

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Thermobacterium Medium

[所属分类:培养基配方] [发布时间:2021-9-7] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

(NH4)2SO4..................................................................................... 1.3g

Yeast extract.................................................................................. 1.0g

Pancreatic digest of casein............................................................ 1.0g

KH2PO4....................................................................................... 0.28g

MgSO4·7H2O ............................................................................ 0.247g

CaCl2·2H2O............................................................................... 0.074g

FeCl3·6H2O ............................................................................... 0.019g

Salt solution ...............................................................................1.0mL

pH 8.5 + 0.2 at 25°C

Salt Solution:

Composition per liter:

Na2B4O7·10H2O............................................................................ 4.4g

MnCl2·4H2O.................................................................................. 1.8g

ZnSO4·7H2O ............................................................................... 0.22g

CuCl2·H2O .................................................................................. 0.05g

Na2MoO4·2H2O........................................................................... 0.03g

VOSO4·2H2O.............................................................................. 0.03g

Preparation of Salt Solution: Add components to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 2.0

with H2SO4.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes in 11.0–12.0mL volumes. Autoclave

for 15 min at 15 psi pressure–121°C. Allow tubes to solidify in a slant

ed position.

Use: For the cultivation and maintenance of Thermomicrobium

roseum.