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Thermobacteroides proteolyticus Medium

[所属分类:培养基配方] [发布时间:2021-9-7] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

NaHCO3........................................................................................ 5.0g

Pancreatic digest of casein............................................................ 2.0g

Yeast extract.................................................................................. 2.0g

MgCl2·6H2O ................................................................................. 1.0g

NH4Cl ........................................................................................... 1.0g

CaCl2·2H2O .................................................................................. 0.4g

K2HPO4......................................................................................... 0.4g

Na2S·9H2O.................................................................................... 0.3g

Resazurin ...................................................................................1.0mg

Trace elements solution ...........................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

MgSO4·7H2O ............................................................................... 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl .............................................................................................. 1.0g

MnSO4·2H2O ............................................................................... 0.5g

CoSO4·7H2O ............................................................................... 0.18g

ZnSO4·7H2O ............................................................................... 0.18g

CaCl2·2H2O................................................................................... 0.1g

FeSO4·7H2O.................................................................................. 0.1g

KAI(SO4)2·12H2O....................................................................... 0.02g

CuSO4·5H2O ............................................................................... 0.01g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·2H2O ......................................................................... 0.01g

NiCl2·6H2O ............................................................................... 0.025g

Na2SeO3·5H2O...........................................................................0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with

KOH. Add remaining components. Add distilled/deionized water to

1.0L.

Preparation of Medium: Prepare and dispense medium under 80%

N2 + 20% CO2. Add components to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Thermobacteroides pro

teolyticus.