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Thermogymnomonas Medium (DSMZ Medium 1141)

[所属分类:培养基配方] [发布时间:2021-9-7] [发布人:网站管理员2] [阅读次数:] [返回]

Thermogymnomonas Medium

(DSMZ Medium 1141)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

KH2PO4......................................................................................... 3.0g

(NH4)2SO4..................................................................................... 0.2g

MgSO4·7H2O ................................................................................ 0.5g

CaCl2·2H2O.................................................................................. 025g

Glucose solution ......................................................................50.0mL

Yeast extract solution ...............................................................50.0mL

pH 3.0 ± 0.2 at 25°C

Glucose Solution:

Composition per 50.0mL:

Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril

ize.

Yeast Extract Solution:

Composition per 50.0mL:

Yeast extract.................................................................................. 1.0g

Preparation of Yeast Extract Solution: Add yeast extract to dis

tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except glucose and

yeast extract solutions, to distilled/deionized water and bring volume

to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to room temperature. Aspetically add glucose and yeast

extract solutions. Mix thoroughly. Adjust pH to 3.0 with sterile 10N

H2SO4.

Use: For the cultivation of Thermogymnomonas spp.