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Thermomicrobium fosteri Agar

[所属分类:培养基配方] [发布时间:2021-9-7] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

NH4Cl ........................................................................................... 2.0g

Na2HPO4..................................................................................... 0.21g

MgSO4·7H2O................................................................................ 0.2g

NaH2PO4..................................................................................... 0.09g

KCl.............................................................................................. 0.04g

CaCl2......................................................................................... 0.015g

ZnSO4·7H2O .............................................................................70.0μg

H3BO3 .......................................................................................10.0μg

MnSO4·5H2O............................................................................10.0μg

MoO3 ........................................................................................10.0μg

CuSO4·5H2O...............................................................................5.0μg

FeSO4·7H2O............................................................................... 1.0mg

Heptadecane, filter sterilized ...................................................20.0mL

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except heptadecane, to

distilled/deionized water and bring volume to 980.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°–55°C. Aseptically add 20.0mL of sterile

heptadecane. Mix thoroughly. Aseptically distribute into sterile tubes.

Cool tubes rapidly in a slanted position.

Use: For the cultivation and maintenance of Thermomicrobium fos

teri.