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Thermophilic Hydrogen-Bacteria Medium

[所属分类:培养基配方] [发布时间:2021-9-7] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1000.5mL:

Na2HPO4·12H2O........................................................................... 4.5g

KH2PO4......................................................................................... 1.5g

NaCl.............................................................................................. 1.0g

NH4NO3 ........................................................................................ 1.0g

MgSO4·7H2O................................................................................ 0.2g

CaCl2·2H2O ............................................................................. 10.0mg

FeSO4·7H2O............................................................................. 10.0mg

Trace elements solution .............................................................0.5mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

ZnSO4·7H2O ............................................................................ 28.0mg

CoCl2·6H2O ............................................................................... 4.0mg

H3BO3 ........................................................................................ 4.0mg

MnSO4·5H2O............................................................................. 4.0mg

MoO3 ......................................................................................... 4.0mg

CuSO4·5H2O.............................................................................. 2.0mg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Incubate cul

tures in 5% O2 + 80% H2 + 10% CO2.

Use: For the cultivation and maintenance of Hydrogenobacter ther

mophilus and Pseudomonas species.