培养基配方

您现在的位置：网站首页 >> 培养基配方

Thermophilic Streptomycete Medium IA

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Sucrose.......................................................................................... 5.0g

Pancreatic digest of casein............................................................ 5.0g

Yeast extract.................................................................................. 3.0g

MgSO4·7H2O ................................................................................ 0.5g

FeSO4·7H2O................................................................................ 0.01g

Dung extract...............................................................................5.0mL

Molasses.....................................................................................5.0mL

Trace elements solution .............................................................1.0mL

pH 7.2 ± 0.2 at 25°C

Dung Extract:

Composition per 100.0mL:

Sheep manure, dried ................................................................... 25.0g

Preparation of Dung Extract: Add dried sheep manure to

100.0mL of tap water. Mix thoroughly. Autoclave for 30 min at 15 psi

pressure–121°C. Filter through Whatman #1 filter paper. Store at 4°C

under toluene.

Trace Elements Solution:

Composition per 100.0mL:

Fe(NH4)2SO4................................................................................. 0.1g

ZnSO4 ........................................................................................... 0.1g

MnSO4 ........................................................................................ 0.05g

CoSO4 ......................................................................................... 0.01g

H3BO3 ......................................................................................... 0.01g

CuSO4 ........................................................................................ 8.0mg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of thermophilic streptomycetes.