编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

Thermoterrabacterium Medium (DSMZ Medium 778)

[所属分类:培养基配方] [发布时间:2021-9-8] [发布人:网站管理员2] [阅读次数:] [返回]

Thermoterrabacterium Medium

(DSMZ Medium 778)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaHCO3...................................................................................... 10.0g

Na2-9,10-anthraquinone-2,6-disulfonate .................................... 8.25g

Yeast extract.................................................................................. 1.0g

KH2PO4....................................................................................... 0.33g

NH4Cl ......................................................................................... 0.33g

KCl............................................................................................. 0.33g

MgCl2·6H2O ............................................................................... 0.33g

NiCl2·6H2O.............................................................................200.0μg

Na2SeO3·5H2O........................................................................120.0μg

Na2WO4·2H2O..........................................................................30.0μg

Calcium chloride solution........................................................10.0mL

Vitamin solution.......................................................................10.0mL

Glycerol (87%) ..........................................................................3.0mL

Trace elements solution SL-10 ..................................................1.0mL

pH 6.8 ± 0.2 at 25°C

Calcium Chloride Solution:

Composition per 10.0mL:

CaCl2·2H2O ................................................................................ 0.33g

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O to

10.0mL of distilled/deionized water. Mix thoroughly. Sparge with

100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

room temperature.

Trace Elements Solution SL-10:

Composition per liter:

FeCl2·4H2O................................................................................... 1.5g

CoCl2·6H2O ........................................................................... 190.0mg

MnCl2·4H2O .......................................................................... 100.0mg

ZnCl2........................................................................................ 70.0mg

Na2MoO4·2H2O ....................................................................... 36.0mg

NiCl2·6H2O.............................................................................. 24.0mg

H3BO3 ........................................................................................ 6.0mg

CuCl2·2H2O ............................................................................... 2.0mg

HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized

water and bring volume to 1.0L. Add remaining components. Mix thor

oughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15

psi pressure–121°C.

Vitamin Solution:

Composition per liter:

Pyridoxine-HCl........................................................................ 10.0mg

Thiamine-HCl·2H2O.................................................................. 5.0mg

Riboflavin .................................................................................. 5.0mg

Nicotinic acid............................................................................. 5.0mg

D-Ca-pantothenate ..................................................................... 5.0mg

p-Aminobenzoic acid................................................................. 5.0mg

Lipoic acid ................................................................................. 5.0mg

Biotin ......................................................................................... 2.0mg

Folic acid ................................................................................... 2.0mg

Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Sparge

with 80% H2 + 20% CO2. Filter sterilize.

Preparation of Medium: Add components, except NaHCO3, calci

um chloride solution, and vitamin solution, to distilled/deionized water

and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to

boiling. Boil for several minutes to dissolve the antraquinone. Cool to

room temperature under an atmosphere of 100% CO2. Add solid

NaHCO3. Adjust pH to 6.8 wtih NaOH. Dispense medium under CO2

into tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to room temperature. Aseptically and anaerobically distribute into

sterile tubes or bottles. Before use, add calcium chloride and vitamin so

lutions from anaerobic, sterile stock solution.

Use: For the cultivation of Thermoterrabacterium ferrireducens.

在线客服

Thermoterrabacterium Medium (DSMZ Medium 778)